Day 2 :
Keynote Forum
Mohammad Magdy El-Metwally
Damanhour University, Egypt
Keynote: New Bioactive Secondary Metabolites from Streptomyces lividans AM
Time : 09:30-10:15
Biography:
Abstract:
During our search for bioactive compounds from actinomycetes, the Streptomyces lividans AM was large scale fermented on rice solid medium, followed by working and purification, affording the new 1-nona-decanoyl, 4-oleyl disuccinate (1), the bacterial new metabolite: filoboletic acid; (9Z,11E)-8,13-dihydroxy octadeca-9,11-dienoic acid (2), and the microbial new metabolite: sitosteryl-3b-D-glucoside (3). This was in addition to further ten known bioactive metabolites: ferulic acid (4), glycerol linoleate, linoleic acid, indol-3-acetic acid methyl ester, 4-hydroxy-phenyl acetic acid, 2-hydroxy-phenyl acetic acid, 3-(hydroxy-acetyl)-indole, indol-3-carboxylic, p-hydroxy-benzoic acid and uracil. The chemical structures of the new metabolites (1-3) were confirmed by extensive 1D and 2D NMR and mass spectrometry, and by comparison with literature data. The antimicrobial activity of the strain extract was studied using a set of microorganisms. The isolation and taxonomical characterization of Streptomyces griseorubens strain ASMR4 is reported as well.
Keynote Forum
Shu-Lin Liu
Harbin Medical University, China
Keynote: Defining natural species of bacteria by a common yardstick: Universal genomic parameter to delineate bacteria into discrete phylogenetic clusters
Biography:
Shu-Lin Liu has his expertise in bacterial systematics and evolution. He was the first in the world to conduct comparative genomic studies on Salmonella bacteria and uncovered a series of genomic evolutionary events, with findings published in PNAS, Journal of Bacteriology, Molecular Biology and Evolution, etc. He teaches microbiology, genomics, evolution and classic Chinese literature. He has an Adjunct Academic position at University of Calgary, Canada and conducted teaching and research. As the Dean of College of Pharmacy, Harbin Medical University, he was active in international collaboration and communication activities and organized a broad range of exchange programs with international institutions including University of British Columbia, University of Calgary, Canada and Purdue University, University of Missouri, Kentucky State University, USA, etc
Abstract:
Bacteria are classified, like higher organisms into species but the current taxonomic species contain bacteria of enormous phylogenetic diversity, causing serious confusions in medical practice and other fields. Therefore, a common yardstick is badly needed for universally defining bacterial species by using a parameter that produces discrete rather than continual data to reveal clear-cut distinctions among the species. Using Salmonella as the primary model to search for such delineating genomic parameters, we found that members of a monophyletic bacterial grouping equivalent to natural species have a high percentage of their common genes sharing identical nucleotide sequences. The percentage windows are mostly broad: >70% for members within a species and <10% for bacteria between species. Similarly, broad percentage windows were also seen in Streptomyces; we propose percentages (<70%) to reflect genetic boundaries and exclude bacteria from a species. The clear-cut nature of such percentages makes them suitable as a common yardstick to define natural bacterial species. The broad percentage windows could be interpreted as the results of non-overlapping gene pools; bacteria of the same gene pool can purge less adapted members once they acquire beneficial traits, but they cannot do that across different gene pools.
Keynote Forum
Stef Stienstra
Dutch Armed Forces/Royal Dutch Navy, Netherlands
Keynote: Zoonotic diseases threat needs sharing of information and new diagnostic systems in less developed countries
Time : 9:00-10:00
Biography:
Works internationally for several medical and biotech companies as scientific advisory board member and is also an active reserve-officer of the Royal Dutch Navy in his rank as Commander (OF4). For the Dutch Armed Forces he is CBRNe specialist with focus on (micro)biological and chemical threats and medical- and environmental functional specialist within the 1st CMI (Civil Military Interaction) Battalion of the Dutch Armed Forces. For Expertise France he is now managing an EU CBRN CoE public health project in West Africa. He is visiting professor at the University of Rome Tor Vergata giving lectures for the CBRN Master study. In his civilian position he is at this moment developing with MT-Derm in Berlin (Germany) a novel interdermal vaccination technology as well as a new therapy for cutaneous leishmaniasis for which he has won a Canadian ‘Grand Challenge’ grant. With Hemanua in Dublin (Ireland) he has developed an innovative blood separation unit, which is also suitable to produce convalescent plasma for Ebola Virus Disease therapy. He has finished both his studies in Medicine and in Biochemistry in The Netherlands with a doctorate and has extensive practical experience in cell biology, immuno-hematology, infectious diseases, biodefense and transfusion medicine. His natural business acumen and negotiation competence helps to initiate new successful businesses, often generated from unexpected combinations of technologies.
Abstract:
Sharing public health threat information is a necessity for governments to prevent outbreaks of infectious diseases. Zoonotic diseases are the most dangerous for outbreaks running out of control, as the population does not have natural nor artificial (from vaccination) immune response to new emerging diseases. The recent Ebola Virus Disease outbreak in West Africa was such an example. New diagnostic methods, which can be performed in developing countries lacking critical infrastructure have to be developed to have an early response on (potential) outbreaks. It must be high tech with high reliability, which can be used in rural areas without proper infrastructure. The mitigation of highly infectious and deadly disease pandemics have to be recognized at the source. Sophisticated diagnostic equipment and good calibration, maintenance and interpretation of the results is essential. To identify pathogens at molecular level new technologies are under development. In developing countries military and civilian actors cooperate fruitfully in fighting potential biological threats. In this civil-military cooperation it is not only the biosafety, which has to be considered, but also the biosecurity, as misuse of extremely dangerous strains of microorganisms cannot be excluded. Several zoonotic infectious diseases, like anthrax, small pox and also the hemorrhagic fevers like Ebola Virus Disease are listed as potential bioweapons. With this extra threat in mind, both biosafety and biosecurity have to be implemented in all mobile or fixed clinical laboratories. An information/computer network with a cloud in which essential information can be traced, helps in early detection of outbreaks of ‘new’, mostly zoonotic, infectious diseases. The same technology helps in the forensic aspects in case of a bioterror attack
Keynote Forum
Clarence M Ongkudon
University Malaysia Sabah, Malaysia
Keynote: Screening of lignin-degrading microorganisms from Sabah Biodiversity for optimum ligninolytic potential
Time : 10:00-10:45
Biography:
Clarence M. Ongkudon who graduated with PhD in Bioprocess Engineering from Monash University, Australia in 2011 is the coordinator of the Bioengineering Research Group (BERG) in Biotechnology Research Institute (BRI) of University Malaysia Sabah, Malaysia. Dr. Clarence has contributed significantly to the field of bioprocess and biochemical engineering where he develops and creates valuable biomolecules from complex cellular materials in the form of therapeutic vectors and products for vaccination and gene therapy application. Dr. Clarence’s most significant contribution to this research field has been the creation of patentable intellectual properties and new knowledge in the field of biomolecule recognition/purification. This has resulted in 2 international patents within the last 5 years. Dr. Clarence has developed an integrated design and downstream process technology that allows a single-stage rapid purification of homogeneous and supercoiled plasmid DNA vaccine on analytical, semi-preparative and preparative scales. This body of work has been a major breakthrough in bioprocess engineering, as purification of plasmid DNA for product development can now be performed rapidly at high throughput with reduced number of unit operations required in downstream processing and increased productivity. This has sparked interests from numerous internationally renowned companies including Boehringer Ingelheim, Qiagen, Promega, Gen Script, Pall, Millipore and Sartorius. Dr. Clarence has published extensively for the past 3 years (over 20 journal and conference publications) in the fields of upstream and downstream processing of therapeutic biomolecules, baculovirus and recombinant proteins. Dr. Clarence aims to create a platform for collaborative projects that work at the cutting edge of biotechnology - drawing together knowledge from medicine, engineering and science in order to tackle biotechnology problems in Malaysia and the world at large.
Abstract:
Lignin is a complex aromatic polymer that intertwining between cellulose and hemicellulose fibers in plant. However, lignin as a by-product during biomass processing is often regarded as nuisance since it retards access to carbohydrates. Recently, there has been much interest in utilization of lignin as petroleum substitutes. In nature, there are diverse groups of microbes that are capable of degrading lignin-rich biomass either in synergistic or competitive manners. Therefore, the use of enzyme cocktails produced from microbial consortia may offer a promising approach to degrade lignin efficiently. The main goal of this research is to search for lignin degrading microbial strains from Sabah biodiversity. Degradation assays to identify suitable isolates for the efficient breakdown of lignin was done on 107 fungi isolates. The results showed that 85 fungi isolates decolorized RBBR (0.01%) effectively compared to Phanarochaete crysosporium. The highest decolorization by F45 with 100% loss of RBBR used. Out of these 85 fungi isolates, a total of 37 and 7 fungi isolates showed higher lignin peroxidase and laccase enzymatic activities, respectively compared to Phanarochaete crysosporium. However, further analysis is required to assess their lignin degrading capability by using real lignin substrate (Kraft lignin).
- Microbiology & Microbes World|Applied Microbiology in Animals |Forensic Microbiology|Petroleum and Biofuels Microbiology|Industrial Microbiology & Food Microbiology|Myocardial infarction and gut micro biota: An incidental connection|Pharmaceutical Microbiology|Gut Microbiota|Oral Microbiology|Molecular Bio robotics
Chair
Maulin P Shah
Enviro Technology Limited, India
Co-Chair
Ewa Solarska
University of Life Sciences in Lublin,Poland
Session Introduction
El Hadji Seydou Mbaye
Cancer Institute, Aristide Le Dantec Hospital, Dakar, Senegal
Title: Human Papillomavirus Infection in genital Women in four regions of Senegal
Biography:
Abstract:
Ahmed G Hegazi
National Research Centre, Egypt
Title: Antibacterial activity of some Saudi Arabia honeys
Biography:
Abstract:
Material & Method: Six Saudi Arabia honeys used to evaluate their antimicrobial activity against some antibiotic-resistant pathogenic bacterial strains with comparison with manuka honey. The bacterial strains were Staphylococcus aureus (Gram positive bacteria), Escherichia coli, Proteus vulgaris, Citrobacter diversus and Salmonella enterica as (Gram negative bacteria).
Hauwa, H
Sokoto State University, Nigeria
Title: BIOSORPTION AND TOLERANCE POTENTIALS OF SOME BACTERIA SPECIES TO HEAVYMETAL FROM CONTAMINATED GOLD MINING SOIL IN ABARE ZAMFARA STATE
Biography:
Abstract:
Soil and water pollution are major global concerns of environmental pollution globally,the release of contaminants into the environment by human activities has increased over the past decades. Soil contamination by mining activities has attracted considerable public attention and the magnitude of the problem in the sampling site calls for immediate action. The use of microorganisms for the recovery of heavy metals from soil sediments has generated growing attention because of the inadequacy and high cost of conventional method of metal treatment technologies. Application of natural and abundant sorption material known as biosorbents comprising of microbial biomass and agricultural waste has drawn attention in the scientific world.
This research was aimed to further exploit the potentials of some bacterial specie for the removal of heavy metal from contaminated mining soils, this bacterial species were isolated from the mining soil and the soil was analyzed for it heavy metal content. The toxic metal of interest for biosorption in this work were lead, cupper, and chromium and the biosorbent used were Bacillus firmus , Bacillus brevis and pseudomonas aeruginosa.
This bacterial species were tested for their tolerance ability in different heavy metal concentration It was recorded that pseudomonas aeruginosa was tolerant to lead at 800mgl-1Bacillus lentus to cupper at 860mgl -1 and Bacillus firmus to chromium at1000mgl-1. The biosorption ability was also studied for 7days and was calculated using beer lambert’s law of biosorption percentage and they all showed good uptake ability with Bacillus firmus removing 96.37% cupper, 93.54% lead and 39.24% chromium. Bacillus brevis remove 97% Cupper, 98% lead and 36% chromium, Pseudomonas aeruginosa was able to absorb 97% each of copper and lead and also 36% of chromium. This is a very important and economic friendly technology for heavy metal bioremediation at cheaper cost.
Ali Reza Ahmadi
Golestan University of Medical Sciences, Iran
Title: Therapeutics Bacteria Against Cancer; Current Advances, Challenges and Opportunities
Biography:
Abstract:
Biography:
Dr. Satish Kumar has expertise in Assisted Reproduction Technology and Applied Microbiolgy.He is Chief Scientist and Head (Research and Development) Auj Innovedic Ayurvedic company Ambala, Haryana, India. He is actively engaged in Research and teaching in Department of Biotechnology. He has guided eleven M.Tech students in different research areas of Biotechnology and has been member and coordinator of various committees of college and university level. D r .Kumar has M.Sc degree in Biotechnology and Molecular Biology , Haryana Agricultural University ,Hisar and PhD in Animal Biotechnology from Chaudhary Devi Lal University , Sirsa , Haryan,India and Authored one Book and Three book chapters Book and Research articles in Journal of International and National Repute. He also serves as member in the scientific advisory board of International Journal of Animal Biotechnology, India. Dr,Kumar is popular speaker who delivered lectures on role of Biotechnology in human welfare and worked with scientists of International repute from top institutions world and and India. He has delivered many lectures in different institutions across country and at international level via webinar on topic “ Advances in Biocatalysis and its impact on early and late development of small molecules to 12 PhD scientists and over 20 Research Associates to Merck Research Laboratories U.S,A. His Students working as Scientist and doing PhDs in US , Canada, Australia etc.
Abstract:
Presently emergence of multiple drug resistance to human pathogenic organisms is serious problem around the world, so development of alternative antimicrobial drugs for the treatment of infectious diseases is the need of hour. One approach is to search for medicinal plants, for possible antimicrobial property. In the present study five solvents viz. ethanol, methanol, chloroform, hexane and water was used for extraction from eleven selected plants and used against E. coli, P. aeruginosa which normally found in diabetic patients while C. albicans found in cancer patients. A total of 55 plant extracts were used in the present study. Antimicrobial activity of plant extract found maximum in Azadirachata sp. followed by Embilica sp., Psidium sp., Citrus sp., Murraya sp., Cannabis sp. and Piper sp. and minimum in Amaranthus sp. and Coriandrum sp. Ethanolic extracts of Azadirachata sp. and Embilica sp. while aqueous extracts of Cannabis sp. and Embilica sp. was most effective against E. coli. Ethanolic extract of Cannabis sp. showed maximum zone of inhibition against P. aeruginosa and methanolic extract of citrus sp. found most effective against C. albicans among eleven selected anticancer and antidiabetic plants. The MIC value of the ethanol extract of most promising plant i.e Azadirachata indica was recorded at 5% (5g/100ml). Results from the present study showed that 95% of ethanol extracts of Azadirachata indica had antimicrobial activity against all tested microorganisms. Antibiotic susceptibility of test microorganisms displayed that imipenem antibiotic has higher zone of inhibition of against E. coli followed by levofloxacin, cefotaxime, aztreonam, ceftazidime and amikacin. Against P. aeruginosa showed maximum inhibition zone followed by cefotaxime, amikacin, imipenem, azetronam and ceftazidime, while maximum zone of inhibition was recorded against C. albicans using ketoconazole followed by miconazole, nystatin, clotrimazole. These antibiotic principles are actually the defensive mechanisms of the plants against pathogens. Laboratory and clinical studies of eleven selected medicinal plants especially the most promising plant extract are required in order to better understand the antimicrobial properties so as to allows the scientific community to recommend their uses as an accessible alternative to synthetic antibiotics.
- Animals in a bacterial world, a new imperative for life sciences |Host pathogen Interaction | Paleomicrobiology, Archaeomicrobiology & Microbial Forensics | Bioinformatics for Micro biome | Geomicrobiology | Industrial Microbiology and Future Bio industries| Prebiotics and Probiotics | Human Micro biota | Public Health & Epidemiology
Location: Radisson Narita
Chair
Maulin P Shah
Enviro Technology Limited, India
Co-Chair
Ewa Solarska
University of Life Sciences in Lublin,Poland
Session Introduction
Jan Bobek
Jan Evangelista Purkyne University, Czech Republic
Title: Awakening of Streptomyces: Novel insights into germination of bacterial arthrospores
Biography:
Abstract:
Lucia Aquilanti
Politecnica delle Marche, Italy
Title: Microbiota and key technological features of naturally fermented Crithmum maritimum sprouts
Biography:
Abstract:
Klaudia Gustaw
University of Life Science in Lublin , Poland
Title: Production of mannitol by novel strain of Lactobacillus hilgardii
Biography:
Abstract:
Jaroslav Budis
Comenius University Science Park, Bratislava, Slovakia
Title: Pheri - Sequence based classification of bacteriophages
Time : 16:00 -16:30
Biography:
Abstract:
Bacteriophages are viral particles that infect and replicate inside bacterial organisms. Since they are specific to a particular strain of bacteria, advances in their research could lead to novel means of targeted treatment without adverse effects on natural microbiome in patient’s body. Moreover, this treatment could be effective against bacterial strains with antibiotic resistance. One of the main bottlenecks of bacteriophage research is inability to cultivate some of the phages due to missing information about their hosts.
We designed a bioinformatics pipeline, called Pheri (Phage Host ExploRatIon), to predict bacteriophage hosts from its genomic sequence. The decision is supported by a set of genes that should correspond to the specificity of the phage. We evaluated the pipeline on a set of 6277 phage sequences downloaded from several publicly available databases.
Our pipeline has a potential to assist in discovery and characterization of novel phages and underlying mechanisms behind their behavior.
Miroslav Böhmer
Comenius University, Science Park, Slovakia
Title: Identification of microbial diversity in grape must by whole metagenome sequencing
Time : 16:30 - 17:00
Biography:
Abstract:
Mohammad Magdy El Metwally
Damanhour University, Egypt
Title: New bioactive secondary metabolites from Streptomyces lividans
Biography:
Abstract:
During our search for bioactive compounds from actinomycetes, the Streptomyces lividans was large scale fermented on rice solid medium, followed by working and purification, affording the new 1-nona-decanoyl, 4-oleyl disuccinate (1), the bacterial new metabolite: filoboletic acid; (9Z,11E)-8,13-dihydroxy octadeca-9,11-dienoic acid (2), and the microbial new metabolite: sitosteryl-3 -D-glucoside (3). This was in addition to further ten known bioactive metabolites: ferulic acid (4), glycerol linoleate, linoleic acid, indol-3-acetic acid methyl ester, 4-hydroxy-phenyl acetic acid, 2-hydroxy-phenyl acetic acid, 3-(hydroxy-acetyl)-indole, indol-3-carboxylic, p-hydroxy-benzoic acid and uracil. The chemical structures of the new metabolites (1-3) were confirmed by extensive 1D and 2D NMR and mass spectrometry, and by comparison with literature data. The antimicrobial activity of the strain extract was studied using a set of microorganisms. The isolation and taxonomical characterization of Streptomyces griseorubens strain ASMR4 is reported as well.
Davinder Singh
Himachal Pharmacy College, India
Title: Synthesis, spectral studies and antimicrobial activity of coumarin derivatives
Biography:
Abstract:
These research study is aimed to synthesize a serious of various substituted derivatives of 8-methyl-2-substituted-6H-chromeno [6, 7-d] oxazol-6-one (6a-6f) and (7a-7b) from 6-Amino-7-hydroxy-4-methyl-2H-chromen-2-one by reaction with different substituted aldehydes and acetic anhydrides in the presence of glacial acetic acid and pyridine. The structure for compounds has been determined by IR, 1H NMR spectroscopy. All the synthesized compounds 1-8 have been screened for their anti-microbial activity with reference drug Ciprofloxacin by using cup-plate method. Among all the synthesized derivatives, compounds which are substituted with 4-phenyl (6a), 4-bromo phenyl (6b), 4-nitro phenyl (6c), 4-chloro phenyl (6d), 2-chlorophenyl (6f) exhibited the most promising antimicrobial activity against Escherichia coli (MTCC 614) and Staphylococcus aureus (MTCC 3160). Coumarin nucleus incorporating oxazole moiety also possess synergism with total eight conventional antibacterial agents, i.e. chloramphenicol (CL), gentamycin (CN), fosfomycin (FF), levofloxacin (LE), minocycline (MI), tazobactam (P/T), teicoplanin (TE), vancomycin (VA), against Methicillin-resistant staphylococcus aureus (MRSA) strains.
Biography:
K M Yacob is a practicing Physician in the field of Healthcare in the state of Kerala in India for the last 30 years and is very much interested in basic research.
Abstract:
When the disease becomes threat to life or organs blood circulation decreases, Temperature of fever will emerges to increase prevailing blood circulation. And it acts as a protective covering of the body to sustain life. When blood flow decrease to brain, the patient becomes fainted-delirious. If we try to decreases temperature of fever, the blood circulation will further reduced. Blood circulation never increases without temperature increase. Delirious can never be cured without increase in blood circulation. The temperature of fever is not a surplus temperature or it is not to be eliminated from the body. During fever, our body temperature increases like a brooding hen`s increased body temperature. The actual treatment to fever is to increase blood circulation. Two ways to increase blood circulation. (1) Never allow body temperature to lose and (2) Apply heat from outside to the body. When the temperature produced by body due to fever and heat which we applied on the body combines together, the blood circulation increases. Then body will stop to produce heat to increase blood circulation. And body will get extra heat from outside without any usage of energy. How can we prove that the temperature of fever is to increase blood circulation? If we ask any type of question related to fever by assuming that the temperature of fever is to increase blood circulation we will get a clear answer. If avoid or evade from this definition we will never get proper answer to even a single question. If we do any type of treatment by assuming that the temperature of fever is to increase blood circulation , the body will accept, at the same time body will resist whatever treatment to decrease blood circulation. No further evidence is required to prove the temperature of fever is to increase blood circulation.