Scientific Program

Conference Series LLC Ltd invites all the participants across the globe to attend 7th World Conference on Applied Microbiology and Beneficial Microbes Tokyo, Japan.

Day 1 :

  • Medical microbiology
Biography:

Abstract:

Aim: This study is aimed at characterization of both antimicrobial and anti-biofilm activity of R-pyocin from clinical Pseudomonas aeruginosa against Gram-positive pathogens including Staphylococcus aureus. Methods and Results: Pyocinogenic P. aeruginosa was detected using reverse-side method, and pyocinogeny typing was confirmed using revised-spotting method. Transmission electron microscopy (TEM) was used for morphological characterization of R-pyocin and for detection of changes in membrane of R-pyocintreated S. aureus. SDS-PAGE analysis was used for detection of the molecular weight of R-pyocin protein-subunits and Poisson-killing-distribution assay for burstsize calculation. Lipotechoic-acid (LTA) adsorption-assay was used to confirm whether LTA in Gram-positive bacteria served as R-pyocin receptor. Moreover, Rpyocin production at 10–60°C was assessed herein. Host-range of activity of Rpyocin was tested against antimicrobial resistant (AMR) pathogens. The anti-biofilm activity of R-pyocin was detected against sensitive bacterial strains. Chemical, enzymatic, pH and thermo-stability of R-pyocin were evaluated. TEM micrographs revealed a typical morphology of myotailocins indicating the production of R-pyocin designated as RPU15. TEM revealed pores formation in S. aureus membrane, and bacteriophage-like plaques were obvious on plates of R-pyocin-treated S. aureus. Rpyocin activity was neutralized by LTA of S. aureus and Listeria monocytogenes. Pseudomonas aeruginosa PU15 produced ~428 non-inducible R-pyocin particles. RPU15 sheath and tube protein-subunits exhibited a molecular weight of 38 and 23 kDa, respectively. RPU15 possessed activity against S. aureus, L. monocytogenes, Bacillus cereus and Candida albicans and reduced biofilm-biomasses of tested AMR strains.

Speaker
Biography:

I have completed my postgraduation at the age of 22 years from Bharathiar university and bachelor’s degree from Mahatma Gandhi university, Kottayam. I have published 4 papers in reputed journals. Now I am doing Phd in microbiology from Manonmaniam sundaranar university,Tirunelveli, Tamilnadu, India .

Abstract:

The development of multi drug resistant uropathogens is a big threat to human race. Infections with multidrug-resistant bacteria are hard to treat. The present study Characterization of bioactive compounds from marine actinomycetes antagonistic to urinary tract bacterial pathogens is aims to prove marine actinomycetes have some bioactive compounds which are antagonistic to multi drug resistant uropathogens. In the present study, the uropathogens were collected from urine samples of patients with their consent. Marine actinomycetes were isolated from marine sediments collected from different stations of Poovar coastal region, part of Arabian Sea on the western coast of India using standard microbiological techniques. The isolated strains were subjected to downstream characterization and those showed significant antagonistic activity common uropathogens were subjected to further studies. The isolate PVR9 showed maximum inhibitory activity against Escherichia coli (19mm). Similarly PVR4 showed maximum activity against Klebsiella sp. PVR35 showed maximum activity against Pseudomonas sp. in well diffusion assay. But in disc diffusion method PVR2 is the most potent strain against Pseudomonas sp. Against Acinetobacter sp. PVR9 showed the highest antagonistic activity in both the secondary assays followed by PVR2. PVR4 is the most potent isolate against Klebsiella sp. in both disc and well diffusion methods. These observations showed lime light on the fact that the marine actinomycetes of the tropical area are a good resource of potential bioactive molecules. Further studies on these isolates will help to combat the multi-drug resistance in clinical scenario.

 

Biography:

Abstract:

Lactic acid bacteria (LAB) can be defined as gram-positive, acid-tolerant, generally non-sporulating, non-respiring, either rod-shaped (bacilli) or spherical (cocci) bacteria that share common metabolic and physiological characteristics. These bacteria are usually found in decomposing animal and plants sources, produce lactic acid as the major metabolic end product of carbohydrate fermentation. They are frequently distributed in traditional fermented foods and beverages and they are known by producing varieties of bioactive compounds which have effective antagonistic activity against food borne pathogens and spoilage microorganism. Lactic acid bacteria are widely used as probiotics in food industry since they are useful in prevention and treatment of diarrheal diseases.  Probiotics are live microorganisms which have effective antagonistic activity against food borne pathogens and if administered in adequate amounts which can promote the health of the consumer. Therefore, this study was aimed to evaluate the antimicrobial activity and probiotic property of lactic acid bacteria isolated from some selected Ethiopian traditional fermented foods and beverages.  Hence, for this study different types of Ethiopian traditional fermented foods and beverages like( Kotcho, Bulla, Ergo, Shamita, Borde and Bukuri) were collected and transported to Microbiology laboratory (Jimma University, Biology)  for  isolation  and characterization, evaluation of antimicrobial and  probiotic property of LAB isolates. Totally about 180 samples; 30 Kotcho, 30 Bulla, 30 Ergo, 30 Shamita, 30 Borde and 30 Bukuri were collected for isolation and characterization of lactic acid bacteria. Isolation and characterization of lactic acid bacteria were conducted following their morphological, physiological, biochemical and molecular characterizations and to evaluate the probiotic activity of LAB, the tolerance and   survival rate of LAB to different stress condition like low pH, intestinal inhibitor substance, high salt concentration, bile salt, stimulated gastric/ intestinal juice and intestinal inhibitor substance were evaluated. Similarly, antimicrobial activity of LAB isolates were investigated against standard bacteria pathogens; S.aureus, S.Thyphrium, E.coli, P.aurugionsa, K.pnemonia and C. albcans.    A total of 360 LAB isolates were identified from Ethiopian traditional fermented foods and beverage, 94(26%) of them were isolated from bulla. About, 125 isolates showed good antagonistic activity against selected food borne pathogens, 18 of the isolates showed 60-94% of survival rate to low pH(2,2.5 and 3), bile salt(0.2 and 0.3%), intestinal inhibitor substances( phenol, bile, low acidity, pepsin and pancreatic), and stimulated gastro-intestinal environments. The capacity of the isolates to survive acids, intestinal inhibitor substance and stimulated intestinal condition, and bile salt tolerance, effective antagonistic activities help to have good and more effective antagonistic activity against all selected food borne pathogens.  All the 18 isolates were resistance to Amplicin, Vancomycin, Gentamycin, Kanamycin, Clindamycin and chloramphenicol, while they were susceptible to Streptomycin and Tetracycline the morphological, biochemical and physiological, carbohydrate utilization characterization and test were performed, accordingly the isolates were grouped under Lactobacillus species(75%), followed by lactococcus and pedioccoccus species. 

All the isolates, 18LAB strains, were found to be the most potential Lactic acid bacteria So, and they were selected as potential probiotic LAB that can be  applied as best starter culture and bio-preservative for the enhancement food shelf life. On other hands, the outcome of these studied parameters were  used as input data for a principal component analysis (PCA) to select the most promising isolate and the isolates were identified through 16S rDNA sequencing. This study provided a basis for the selection of antimicrobial peptides and the development and utilization of LAB for their potential antimicrobial activity and probiotic activity.