Hyun Gee Lee has completed his Master's degree at the age of 26 years from Yonsei University. He is currently a researcher at AMOREPACIFIC.
Oral hygiene products having various kinds of calculus inhibitory effects are being introduced on the market. However, there is no mention of the effects on microorganisms in oral hygiene products that have the effect of inhibiting dental calculus formation. The object of this study is to identify the causative microorganisms that influence dental calculus production. A total of 80 calculus patients are recruited. After scaling initially, two groups were randomly assigned the toothpaste according to the presence of calculus inhibitors. Allow 90 days of use and perform microbial analysis before and after use. Microbial community analysis was performed using NGS (Next Generation Sequencing). Principal coordinate analysis (PCoA) was performed to characterize the overall characteristics of the dental calculus-inducing microbiome before and after product use. In the control group, it was confirmed that there was no significant difference in the bacterial community composition before and after using the product in both weighted and unweighted UniFrac distance. The test group showed significant differences in bacterial community composition before and after using the product in both weighted and unweighted UniFrac distance. A linear discriminant analysis effect size (LEfSE) analysis was performed to identify bacteria with significant differences before and after use of the product. Haemophilus parainfluenzae was identified as a bacterium that causes dental calculus formation. Streptococcus has been identified as a normal oral flora. When dental calculus formation inhibitors were used, the changes in oral microbiome were identified and Haemophilus parainfluenzae was identified as a bacterium that can induce dental calculus formation.