Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 4th International Conference on Applied Microbiology and Beneficial Microbes Tokyo, Japan.

Day 1 :

Conference Series Applied Microbes - 2019 International Conference Keynote Speaker Lidia Sas Paszt photo

Organization: Department of Soil and Orchard Management, Rhizosphere Laboratory (IO). Experience in research projects and R&D projects: CEAF, CRAFT, EraNet RURAGRI, HortiEnergia, Huntsman, EFSA carried out in collaboration with academic and industrial partners in Poland and abroad. Coordinator and executor of tasks of the research and development project EkoTechProdukt co-financed by the European Commission from the European Regional Development Fund, (2009-2015). Research interests & Achievements in implementation of R&D results and innovative activities: studies in the area of rhizosphere and nutrient management strategies in fruit crops, development of microbial inocula for horticultural production, development of bioproducts for fruit crops. Achievements in the field of industrial property rights: patents, patent applications: development of 6 new microbial consortia and 5 new bioproducts for use in organic fruit growing. Other achievements: member of the WGs of Cost Action 836 (2002-2004), member of the MCs of 3 COST Actions: 631 (2002-2005), E38 (2005-2008), TD 1107 (20012-2016), expert representing Poland in the Programme Committee of the European Commission in FP6 (2002-2006) and in the Programme Committee of the 7th EU Framework Programme (2007-2012), expert of EFSA – European Food Safety Authority (2007-2012), member of the Management Committee and Working Group of COST Action ES 1406 ‘Soil fauna - Key to Soil Organic Matter Dynamics and Modelling (KEYSOM)’ (2015-2018), expert evaluating research projects/reports of the 6th and 7th EU Framework Programmes (2002-2013) and Horizon 2020 (2014-2020)


The experiment in stoneware pots was established in the spring of 2018 in four replications in the Experimental Field of the Warsaw University of Life Sciences in Skierniewice. The objects studied were strawberry plants of the cultivar ‘Marmolada’.

The experiment was conducted in stoneware pots with a diameter of 40 cm filled with about 270 litres of arable soil with a pH of 6.2, to each of which three seedlings of the Frigo A+ type (15-18 mm) strawberry plants were planted out at the beginning of May. Each combination consisted of six replications (stoneware pots). The experiment was established in a random block design with a total of 13 experimental combinations, including beneficial microorganisms and filamentous fungi, and a no-treatment (zero) control. In addition, the ‘Marmolada’ strawberry plants were divided into two experimental groups: in one of them the plants were grown in optimal irrigation conditions (100% of the water dose), and in the other, from mid-June, under drought stress (50% of the water dose). In both groups, the same fertilization was applied. The experiment included the following experimental combinations: 1. Control – plants not fertilized; 2. Standard NPK fertilization; 3. Control with the addition of filamentous fungi (Aspergillus niger and Paecilomyces lilacinus); 4. Control – with the addition of beneficial Bacillus bacteria (Bacillus sp., Bacillus amyloliquefaciens and Paenibacillus polymyxa); 5. Standard NPK + filamentous fungi; 6. Standard NPK + beneficial bacteria; 7. 100% Polifoska 6 + beneficial bacteria; 8. 100% Urea + filamentous fungi; 9. 100% Polifoska 6 enriched with beneficial bacteria; 10. 100% Fos Dar 40 enriched with beneficial bacteria; 11. 60% Urea enriched with filamentous fungi; 12. 60% Polifoska 6 enriched with beneficial bacteria; 13. Fos Dar 40 in a 60% dose enriched with three strains of beneficial bacteria.

In 2018, yielding was not assessed because all inflorescences were removed. The amounts of minerals (macro- and micronutrients) were determined in strawberry leaves. In the autumn, runners were collected for assessment, where the number, fresh weight, length, the number of runner plants and their fresh weight were determined. The results of the first year of the study showed that filamentous fungi and beneficial bacteria applied together with mineral fertilizers tested on strawberry plants (Urea, Polifoska 6, Fos Dar 40) increased their biological activity. This was particularly evident in the development of the aboveground parts of strawberry plants (runners and runner plants, and the concentration of some minerals, macro- and microelements, in the leaves).

The amount of water supplied to the strawberry plants fertilized with various mineral fertilizers enriched with beneficial bacteria and fungi had a greater impact on the growth characteristics of the aboveground parts of the plants when using the full dose of water (100%) than the dose of water reduced by half (50%).

The microbiological analysis of the soil showed that the microbially enriched mineral fertilizers Polifoska 6 and Fos Dar 40 (with the addition of beneficial Bacillus bacteria: Bacillus sp., Bacillus amyloliquefaciens and Paenibacillus polymyxa), and Urea at 100% applied together with filamentous fungi (Paecilomyces lilacinus, Aspergillus niger) had a favourable effect on increasing the numbers of beneficial microorganisms in the rhizosphere soil of ‘Marmolada’ strawberry plants.

"This paper is financed by The National Centre for Research and Development in frame of the project BIOSTRATEG, contract number BIOSTRATEG3/347464/5/NCBR/2017".



Keynote Forum

Marina Sidorenko

Far Eastern Branch of Russian Academy of Sciences,Russia

Keynote: Microbiomes for the disposal of chicken manure
Conference Series Applied Microbes - 2019 International Conference Keynote Speaker Marina Sidorenko photo

In 2004, Marina Sidorenko defended her thesis in two specialties: soil science and microbiology. She is a leading researcher at the Federal Scientific Center of the East Asia Terrestrial Biodiversity Far Eastern Branch of Russian Academy of Sciences (FSCEATB  FEB RAS). She has published over 35 articles in well-known journals and 4 patents of the Russian Federation, is a member of the editorial board and reviewer of several journals. Her areas of interest are applied microbiology, soil microbiome, sanitary microbiology.



The intensive development of the poultry industry is associated with the problem of waste management and creates a number of problems. Promising is the biological method using microorganisms. There are many different microorganisms in manure, some of which are “useful”, as they are actively involved in the decomposition of organic substances. At the same time, manure is a source of transmission of a large number of pathogens of animals and humans (according to WHO). Therefore, it is important to develop biotechnological processes for the disposal of organic waste, ensuring the organization of effective, waste-free and environmental technologies for the bioconversion of manure and litter. We conducted a screening of a large number of microorganisms from various systematic groups for the ability to assimilate organic substances, to show enzymatic activity. Microorganisms are also tested for the ability to show bactericidal and bacteriostatic activity, antiparasitic activity. As a result, active strains of microorganisms were selected, from which effective consortia were formed. These consortia are recommended for processing poultry manure to further use the compost as a bio-fertilizer. Such compost contains a sufficient amount of basic mineral substances, increases the microbial mass of the soil itself and increases the respiration of the soil. Another direction of use of the substrate obtained by us is its burning, since poultry manure has a high calorific value, and the ash obtained by burning is a complex fertilizer with a high nutrient content.


Conference Series Applied Microbes - 2019 International Conference Keynote Speaker Ewa Solarska photo

Prof. Ewa Solarska, professor and head of the Organic Food Laboratory of Plant Origin  at the Department of Biotechnology, Microbiology and Human Nutrition at the University of Life Sciences in Lublin (UPL). At the university she is also the head of the research team to evaluate chemical and biological plant protection products for registration purposes. Prof. Solarska has a records of research in the area of plant pathology and food quality. She is the author and co-author of about 120 publications and monographs, she has completed about 30 R&D projects (founded by NCBiR, Ministry of Agriculture and Rural Development, Polish Agency for Enterprise Development). Professional experience of prof. Solarska includes, among others, development of organic hops production technology, development of agrotechnical methods for control of hop Verticillium wilt, detection of pathogenic fungi, viruses and viroids in plants by molecular methods, obtaining of hops free from viruses and viroids, development of cereal, fruits and vegetables cultivation technology in the organic system, research on the effectiveness of natural plant protection products, determination of the quality of organic cereals and their products in terms of content of mycotoxins, estimation of biological and chemical products efficacy against pests, weeds and diseases of different crops for registration aims. Professor received international awards and national awards, among others, for his scientific merits: the PAN award for outstanding scientific achievements in the field of commercialization of research results, twice the Minister of Agriculture and Rural Development award for the development and dissemination of organic hop cultivation technologies and new bioproducts. These studies concerned the evaluation of the effectiveness of probiotic microorganisms and plant extracts in control of diseases and pests of this plant, as well as organic fertilizers and basalt powder in enriching the soil with organic matter and improving the quality of hops.  Under her supervision, 3 doctoral thesis was made. Currently she is scientific supervisor of the 3 PhD students. The subject of these doctoral thesis concerns toxinogenic fungi of the genus Fusarium, yeasts inhibiting fungi of the genus Fusarium, as well as a comparison of the microbiome of  plants from organic and conventional farming.


Microorganisms play an important role in plant health protection. Some of them are known as producers of bioactive substances such as vitamins, hormones, enzymes, antioxidants and antibiotics that can directly or indirectly enhance the growth and protection of plants. The use of microorganisms in consortia allows their better survival in any environment compared to single-species preparations, due to their synergism and the creation of a biofilm that is more effective for consortia with more efficient metabolism and more resistant to oxidative stress, as well as for toxic factors. Beneficial  microorganisms that increase growth and protect plants can be added to the soil to improve the health of plants. The reason for this phenomenon is the close dependence of plants on their microbiome. By gaining better control over these relationships, pest growth can be reduced and the ecosystem can be more stable. Also fermented plant extracts that enhance plant defense mechanisms or directly toxic effects are used to increase the effectiveness of microorganisms in  plant protection.


Keynote Forum

Maulin P Shah

Enviro Technology Limited, India

Keynote: :Industrial Waste Water Treatment
Conference Series Applied Microbes - 2019 International Conference Keynote Speaker Maulin P Shah photo

Maulin P. Shah, currently Chief Scientist & Head – Industrial Waste Water Research Lab, Division of Applied and Environmental Microbiology Lab at Enviro Technology Ltd., Ankleshwar, Gujarat, India, received his Ph.D. (2002-2005) in Environmental Microbiology from Sardar Patel University, Vallabh Vidyanagar, Gujarat. He has served as an Assistant Professor at Godhra, Gujarat University in 2001. He is a Microbial Biotechnologist with diverse research interest. A group of research scholars is working under his guidance on the areas ranging from Applied Microbiology, Environmental Biotechnology, Bioremediation, and Industrial Liquid Waste Management to solid state fermentation. My primary interest is the environment, the quality of our living resources and the ways that bacteria can help to manage and degrade toxic wastes and restore environmental health. Consequently, I am very interested in genetic adaptation processes in bacteria, the mechanisms by which they deal with toxic substances, how they react to pollution in general and how we can apply microbial processes in a useful way (like bacterial bioreporters). One of our major interests is to study how bacteria evolve and adapt to use organic pollutants as novel growth substrates. Bacteria with new degradation capabilities are often selected in polluted environments and have accumulated small (mutations) and large genetic changes (transpositions, recombination, and horizontally transferred elements). His work has been focused to assess the impact of industrial pollution on microbial diversity of wastewater following cultivation dependant and cultivation independent analysis. His major work involves isolation, screening, identification and Genetic Engineering of high impact of Microbes for the degradation of hazardous materials. He has more than 200 research publication in highly reputed national and international journals. He directs the Research program at Enviro Technology Ltd., Ankleshwar. He has guided more than 100 Post Graduate students in various disciplines of Life Science. He is an active Editorial Board Member in more than 200 highly reputed Journal’s in the field of Environmental & Biological Sciences. He was Founder Editor-in-Chief of International Journal of Environmental Bioremediation and Biodegradation (2012-2014) as well as Journal of Applied and Environmental Microbiology (2012-2014) (Science and Education Publishing, USA).  He is also serving as a reviewer in various journals of national and international repute. Recently, he has been awarded as a Young Biotechnologist Medal by Biotechnological Society of Nepal”. He is Associate Editor in BMC Microbiology (Springer Nature), Advisory Board in CLEAN-Soil, Air, Water (Wiley), Editor in Current Pollution Reports (Springer Nature), Editor in Bulletin of Environmental Contamination & Toxicology (Springer Nature), Editor in Environmental Technology & Innovation-ELSEVIER, Editor in Current Microbiology-Springer Nature, Editor in Journal of Biotechnology & Biotechnological Equipment-Taylor & Francis, Editor in Ecotoxicology (Microbial Ecotoxicology)- Springer Nature.



This presentation shows the pros and cons of using the combination of various technologies for industrial waste water treatment plant. Rapid industrialization, intensive agriculture and other human activities cause soil degradation,  pollution and lowers the productivity and sustainability of the crops that further increase the pressure on natural resources and contribute to their degradation. Environmental bio remediation is an effective management tool for managing the polluted environment and in restoring the contaminated soil. The use of microbial sources, coupled  with advanced technology is one of the most promising and economic strategies for the removal of environmental pollutants. There is a strong scientific growth with both the in situ and ex situ ways of bio remediation, in part due increased use of natural damping as most of the natural attenuation is due to bio degradation. The degradation of pollutants by environmental bio remediation technology, can be a lucrative and environmentally friendly alternative. This article provides an overview of the important environmental bioremediation technologies and their application
in treating the industrial waste water.


Conference Series Applied Microbes - 2019 International Conference Keynote Speaker Tadashi Shimamoto photo

Tadashi Shimamoto has completed his PhD from Okayama University, Japan and Postdoctoral studies from University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, USA. He is a Professor of Laboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, Japan. He has published more than 80 papers in reputed journals.


Carbapenems are a class of highly potent antibiotics that are commonly used as last-resort antibiotics for treatment of severe infections caused by multidrug-resistant bacteria. Therefore, carbapenem antibiotics are not licensed for foodproducing animals in many countries, only for human use. This study was designed to elucidate the incidence and molecular characterization of foodborne carbapenemases producing bacteria in Japan. A total 28 meat samples were collected from local groceries in Higashi-Hiroshima city, Hiroshima, Japan and tested for carbapenemases-encoding genes. Interestingly, 17 bacterial isolates were recovered and only one isolate confirmed to harbor both blaNDM-1 and blaVIM-1. To the best of our knowledge, this is the first report of carbapenem-resistant Klebsiella pneumoniae isolated from food in Japan that produces NDM-1 and VIM-1 carbapenemases. The strain was resistant to various antibiotics and harbored blaNDM-1, blaSHV-71,
blaCTX-M-15, blaTEM-1, qnrS-1, and aac (6')-1b as well as two class 1 integrons: One containing blaVIM-1 and the other aadB-aadA2 and belongs to Sequence Type (ST) 30. In addition, blaNDM-1 was carried on an untypeable self-transmissible plasmid >90 kb in size. Our results are of a great interest and urge the authorities to consider the food as a potential source for carbapenemases and to comprise it for carbapenemase-producing organism’s surveillance programs.

Keynote Forum

Stef Stienstra

Dutch Armed Forces/Royal Dutch Navy, Netherlands

Keynote: The threat of zoonotic diseases and Ebola Virus Disease specifically
Conference Series Applied Microbes - 2019 International Conference Keynote Speaker Stef Stienstra photo

Works internationally for several medical and biotech companies as scientific advisory board member and is also an active reserve-officer of the Royal Dutch Navy in his rank as Commander (OF4). For the Dutch Armed Forces he is CBRNe specialist with focus on (micro)biological and chemical threats and medical- and environmental functional specialist within the 1st CMI (Civil Military Interaction) Battalion of the Dutch Armed Forces. For Expertise France he is now managing an EU CBRN CoE public health project in West Africa. He is visiting professor at the University of Rome Tor Vergata giving lectures for the CBRN Master study. In his civilian position he is at this moment developing with MT-Derm in Berlin (Germany) a novel interdermal vaccination technology as well as a new therapy for cutaneous leishmaniasis for which he has won a Canadian ‘Grand Challenge’ grant. With Hemanua in Dublin (Ireland) he has developed an innovative blood separation unit, which is also suitable to produce convalescent plasma for Ebola Virus Disease therapy. He has finished both his studies in Medicine and in Biochemistry in The Netherlands with a doctorate and has extensive practical experience in cell biology, immuno-haematology, infectious diseases, biodefense and transfusion medicine. His natural business acumen and negotiation competence helps to initiate new successful businesses, often generated from unexpected combinations of technologies.



Public health systems are not always prepared for outbreaks of infectious diseases. Although in the past several public health institutes, like the French ‘Institut Pasteur’ and the Dutch ‘Tropeninstituut‘, were prominent surveyors of infectious diseases, the investments in worldwide public health have decreased. Now more attention is given to curative healthcare compared to preventive healthcare. The recent Ebola Virus Disease outbreak in West Africa initiated a new wave of interest to invest in Worldwide Public Health to prevent outbreaks of highly contagious diseases. Zoonotic diseases are threatening as the population does not have natural nor artificial (from vaccination) immune response to new diseases like in the Ebola Virus Disease outbreak in 2014. The new strain of the Ebola Virus in West Africa was slightly less lethal, compared to other Ebola Virus strains, but the threat of spreading was far bigger as it had a longer incubation time. Most public health systems are not trained well enough to mitigate highly infectious and deadly disease outbreaks. NGO’s helping to fight the outbreak are often better trained in curative treatments and have less experience with biological (bioweapon) threats for which the military are trained for. The UNMEER mission was unique in this. It was a setting in which military and civilian actors cooperate in fighting a biological threat. Protection is essential for health workers. Smart systems have to be developed to prevent further spreading of the disease, but it is not only the biosafety, which has to be considered, but also the biosecurity, as misuse of extremely dangerous strains of microorganisms cannot be excluded. Several zoonotic infectious diseases, like anthrax, smallpox and hemorrhagic fevers are listed as potential bioweapons. Therefor both biosafety and biosecurity have to be implemented in all measures to fight outbreaks of highly infectious diseases.